• SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . 2. 2. It features a modular design that allows users to add or change detection cartridges according to their needs. The fluorescence spectra was obtained on a Spectra Max i3x Multi-Mode Detection Platform. DNA/RNA concentration is commonly estimated by dividing the A260 value by the 1-cm absorptivity value (or multiplying by its reciprocal). Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. The SpectraMax i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6 - 384 well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. at 540 nm using a Spectra Max i3x microplate reader (Molecular devices, San Jose, CA). Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. The SpectraMax i3x reader has variable bandwidth for fluorescence and luminescence, and you are able to choose either 9nm or 15 nm for excitation and either 15 nm or 25 nm for emission. Ferguson, Ph. flThe SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. The SpectraMax i2X is used for cell migration, cell. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Specifically, we present neurite. The intensity of the emitted light can be measured easily with the SpectraMax i3x reader. Example: [Drawer]. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. The plate was shaken and incubated in the dark for 10 minutes. SpectraMax iD5:Fluorescence was quantified using a microplate reader (SpectraMax i3x, Molecular Devices, CA, USA) with an excitation and emission wavelength being 365 nm and 450 nm, respectively (Jian et al. Category 2 and 3 microplate readers use gradient heating as well. The difference in absorbance observed in the presence and absence of SOD was considered to be the amount of superoxide produced, and results. Reduce background noise The cooled PMT reduces background noise allowing for a more sensitive, wide dynamic range in extremely low light. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The PMT gain can be set to manual, high, medium, low, or automatic (Figure 3A). Examples: fiPress carefully to engage,fl fiDo not press down. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. The absorbance at 450 nm was read with a Spectra Max i3X microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA), and the data at 30–40 min post-treatment were analyzed. The SpectraMax i3x reader provides theAbsorption was measured at 490 nm by utilizing a Spectra max i3x Multimode microplate reader (Molecular Devices). User guide for SpectraMax M5 Microplate Reader Before beginning, you should contact Tatiana Laremore (tnl1@psu. 2 x 10-14. , Fisher Scienti fi c, cat. SPECTRAmax 190 Microplate Spectrophotometer Operator™s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax 190 control panel are shown in boxed Helvetica type. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyWe would like to show you a description here but the site won’t allow us. polarization performance of the FilterMax®, SpectraMax® Paradigm®, and select cartridges or read modes of the SpectraMax i3, i3x, and iD5 readers. D. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Find regional support. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. The fluorescence spectra were acquired using a microplate reader (Spectra Max i3x; Molecular Devices, USA). applications, including dual luciferase and ATP assays. The SpectraMax i3x microplate reader measures absorbance, luminescence and fluorescence as standard, and provides the flexibility to add novel. If desired, a high-range standard curve may be prepared from 1 ng/mL to 1 μg/mL, or a low-range standard curve may be prepared from 25 pg/mL to 25 ng/mL. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. 313 reference gain check fail. Supplier: Molecular Devices. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Unpacking and setting up the instrument includes the following procedures: • Unpacking the instrument and saving the original packaging. HTRF IP standard curve. 01 0. Singlet oxygen (1 O 2) generation was determined through 1,3-diphenylisobenzofuran (DPBF) quenching. The calculated LOQ values were 0. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. The SpectraMax ® i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Patented user-exchangeable cartridge design expands the system's detection capability making it. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. 99（图一）。The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. SpectraMax i3x and Paradigm readers require the FP-FLUO. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring SpectraMax i3 Install Guide; SpectraMax i3; SpectraMax i3x; SpectraMax iD3; SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 蛍光 発光 ELISA. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Multiple automated tests are provided including: • Linearity • Precision • Accuracy • Dynamic range • Alignment • Signal to noise • Signal to background • Filter checks iD5. Because microplate readers are meant to shoulder routine and time-consuming tasks for researchers, ease of use is an important feature. See5039228D December2018 SpectraMax® i3x Multi-ModeDetectionPlatform WiththeSpectraMax® MiniMax™300ImagingCytometer UserGuideMolecular Interactions. Molecular Devicesの全商品を見る. Increased fluorescence performance. Itemploysa luciferaselabel(where“label”generallymeansfusedbytheuseofexpressionvectors)forthe oneproteinandaGreenFluorescentProtein(GFP)variantlabelfortheother. 200-800 nm using Spectra Max i3X microplate reader. The SpectraMax® i3 Platform from Molecular Devices® is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Table 1. The SpectraMax® i Series Multi-Mode Mode Microplate Readers offer the ideal solution for any application or budget. The SoftMax Pro Software provides integrated instrument control. The SPECTRAmax 340PC can mea- Learn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. It allows you to explore cellular . The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Thetypeof. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. 635. Quick imaging and analysis of cells gives you a front-row view of the phenotypic changes that accompany. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. 0´ 104 8. The corrected absorbance is within 5% of the value. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. It also integrates with the. Find regional support. The SpectraMax i3x reader provides the. Use the 3. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. pathways and protein activation and expression in one system. 312 gain calibration failed. 306 plate air cal fail,low light. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Order Now. Spectranet customer portalThe SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Equipped with transmitted light (TL), green (Ex/Em: 460/541) and red (625/713) fluorescence channels, the MiniMax cytometer with SoftMax® Pro Software can be used to image and. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Average 98 stars, based on 1 article reviews. 314 low lamp level warning. VersaMax, SpectraMax 340PC384, 190, Plus 384 Microplate Readers User Guide Electromagnetic Compatibility Regulatory for Canada (ICES/NMB-001:2006) This ISM device complies with Canadian ICES-001. The TUNE detection cartridge uses a breakthrough monochromator design that enables researchers to easily optimize their. Bioz Stars score: 98/100, based on 1 PubMed citations. Note: Additional readers with similar performance are listed. The SpectraMax® i3 Platform's base system features an integrated optical system enabling top and bottom reads for 6-, 384- well microplates and launches with three broad detection modes: luminescence, absorbance, and fluorescence. After incubation at 37 °C and 5% CO 2 for 20 min, fluorescence images were taken using a Spectra Max i3x with MiniMax 300 Imaging Cytometer (Molecular Devices, San Jose, CA, USA) with the excitation and emission setup provided with SoftMax Pro 7. The Relative Luminescence Units (RLU) were measured using the SpectraMax i3x Luminescence Glow cartridge (Lum 384) (0200-7015POS, Molecular Devices) and normalized to the cell count or area, which were determined using the Mini MaxTM 300 Imaging Cytometer (5024062, Molecular Devices). The user guide also covers the features and specifications of the SpectraMax MiniMax 300. Be Future Ready with the SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax® 190 spectrophotometer is ideal for most life science applications, especially DNA analysis. Spectranet customer portalWe would like to show you a description here but the site won’t allow us. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. . Also refer to this Multimode Reader Comparison Chart. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 0112-0115G October2018 SpectraMax®M3 SpectraMax®M4 SpectraMax®M5 SpectraMax®M5e Multi-ModeMicroplateReaders UserGuideSpectraMax® Microplate Readers and SoftMax® Pro Software. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. View online or download 1 Manuals for Molecular Devices SpectraMax i3x. Microplate readers, multi-mode, SpectraMax® i3x. Red and green fluorescence was then measured (emission at 590 and 530 nm, respectively, with excitation at 490 nm) using a fluorescence microplate reader (Spectra Max i3x, Molecular Devices Inc. ZERO BIAS - scores, article reviews, protocol conditions and more. This assay uses two fluorescent dyes, calcein AM (cal AM) and ethidium. Unlock your i3/i3x instrument after shipment. XPS spectra were obtained from PHI Versaprobe II. ZERO BIAS - scores, article reviews, protocol conditions and moreSpectraMax i3x offers all the techniques you need to explore cell pathway in a single system. the SpectraMax Paradigm Multi-Mode Detection Platform User Guide. Increased fluorescence performance. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. Bioz Stars score: 98/100, based on 1 PubMed citations. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. Art-science interactive light show/animation. SpectraMaxi3Multi. Description 1. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,The SpectraMax® M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck® Pathlength Measurement Technology:. We would like to show you a description here but the site won’t allow us. Key features. Home > Search Results > Danaher Inc > spectramax i3x. Table 1. Replicate values were averaged and imported into Design-Expert 8. 06, 0. *On the SpectraMax i3x reader only. Learn how you can perform flash-based applications, such as dual luciferase and ATP assays, with high precision and accuracy using this cartridge. Products : FlexStation 3 reader, SpectraMax M5/M5e reader, SpectraMax M2/M2e reader. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. User-upgradeable detection modules allow you to expand the capabilities of your microp. Expand your lab’s capabilities to include flash applications such as dual luciferase and. 2 (Molecular Devices, San Jose, CA). The SpectraMax i3x reader is engineered to perform with Spectral Fusion Illumination for increased sensitivity across the entire wavelength range, and a cooled photomultiplier tube (PMT) for improved detection in extremely. Wokingham, Berkshire RG41 5TS United Kingdom. 315 can't find zero order. Videos & Webinars SpectraMax i3x - Detection Module ChangeSpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Find regional support. Figure 4. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthThe SpectraMax i3x reader grows with you as your research areas expand. TNF-alpha quantification down to low picomolar levels on the SpectraMax Paradigm Platform. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. edu) to be added as a trained user in iLab. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. SpectraMax i3x and Paradigm readers require the FP-FLUO detection cartridge. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. QBT Fatty Acid Transporter Assay. Spectral Fusion Illumination is a powerful combination of Xenon flash lamp and LEDs that provides unmatched signal strengthCapture flash assays with ease The SpectraMax i3x Injector Cartridge with SmartInject® Technology expands your research capabilities to include flash-based applications including dual luciferase and ATP assays. Capture Flash Assays with Ease. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. 2 in. The protocols are also available on the SoftMax Pro Software protocol sharing. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. These concentrations are then used to perform western blots. Carbonyl assay The plates for carbonyl detection were prepared as described. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. The plate was shaken and incubated in the dark for 10 minutes. Bookings are free, but you will need to use iLab to book. Multimode Readers Comparison Chart. Figure 4. 2 (Molecular Devices, San Jose, CA, USA). SpectraDrop Abs Protein Quant (i3-i3x)Molecular Devices SpectraMax i3x Multi-Mode Detection Platform. 303 unable to cal dark current. 제품. Relative mitochondrial polarization was expressed as the ratio of emission at 590 nm to that at 530 nm (Shin et al. Avantor®, a Fortune 500 company, is a leading global provider of mission-critical products and services to customers in the biopharma, healthcare, education & government, and advanced technologies & applied materials industries. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. SpectraMax M 시리즈 Microplate Reader, Cuvette Port, IQ OQ 검증 | Molecular Devices. Recommendations for SpectraMax i3/i3x plate readers The Molecular Devices SpectraMax i3/i3x allows use of the pH-Xtra Glycolysis Assay with time-resolved fluorescence (TRF) detection. Item SpectraMax i3x Multi-Mode Microplate Readers; Company Molecular Devices LLC; Catalog Number SpectraMax i3x <Disabled> This product is no longer available on Biocompare. 0. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. The assay window spanned four orders of magnitude. 0. Settings not required by a reader are indicated by ‘---’. Find regional support. 2. Enzyme activity was presented as absolute activity in a unit of nmol g −1 dry soil h −1. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. SpectraMax M5. Fluorescence was measured using a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA) with 365 nm excitation and 450 nm emission filters. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a cellular pathway. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. CHO M1WT3 cells are Chinese hamster ovary cells stably transfected with the M1 muscarinic G q -coupled receptor. i3x. The drawing shows a standard microplate of 15 mm height covered with a lid. The assay can accurately test the toxicology of compounds as shown in Figure 1. Spectranet customer portal The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. The SpectraMax i3x reader provides the. The SpectraMax MiniMax 300 Imaging Cytometer option can be added at any time to the SpectraMax i3x System, providing an expandable platform that evolves with your changing needs. Enzyme Activation Analysis;. The SpectraMax ® i3x Platform from Molecular Devices is a multi-mode microplate reader that evolves with your future needs and offers an unlimited breadth of application possibilities. AlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. The FRET ratio is defined as the relative fluorescence intensity of an acceptor normalized to the total fluorescence intensity. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. 6% for the on-board optics (monochromators). Published Dec 16, 2020 | Updated Oct 10, 2023. 305 reference level saturation. Briefly, after exposed to ZnO/GO and Cd, cells were collected and washed with ice-cold PBS twice and then re-suspended in 200 μL binding buffer. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. HTRF IP standard curve. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . We would like to show you a description here but the site won’t allow us. Download PDF. Features. 4. UV-Vis absorbance, fluorescence and luminescence • Accommodates plate types from six to 384-well format • Easily upgradeable with interchangeable filter cubesSpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Materials and methodsWe would like to show you a description here but the site won’t allow us. SpectraMax® i3x Multi-Mode Microplate Reader with MiniMax™ imaging cytometer Integrated multi-mode microplate reader and imaging system • Ideal for cell counting, label-free imaging, and cell proliferation • Western blot, imaging, and injectors on one configurable microplate reader • Stain free, automated cell countingusing the SpectraMax DuoLuc Reporter Assay Kit on the SpectraMax i3x microplate reader. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Fluorescent Polarization: Solid black plates, as above. The ratio of the two fluorescence intensities 520/620 (acceptor/donor) enables the calculation of Delta F (%) which. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. The SpectraMax ® i3x. Download PDF. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles). Native or oxidized AN (40 μM) and FN (0. 08, 0. no. Multimode Reader Comparison Chart. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. Microplate optimization should be performed the first time the assay is run, and subsequently for . SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating monochromator which allows you to specify a precise wavelength, from 340 nm to 850 nm, for the absorbance maximum of your sample. Whether you are looking for an intuitive, easy-to-use reader for your busy, multi-user lab, or an upgradeable system that can adapt to support your lab’s new projects and changing objectives, the i Series has you covered. china@moldev. Example: [Drawer]. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Published Dec 16, 2020 | Updated Dec 16, 2020. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. See more, and do more, with the SpectraMax® i3/i3x Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick imaging and analysis of cells and gives you a front-row view of phenotypic changes that accompany cytotoxicity,. Both Microplate Optimization and Read Height Adjustment were. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. com. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. For SpectraMax iD5, i3x, and Mini readers, the read height setting should be optimized for the microplate used. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataBased on the SpectraMax i3x system, ACROBiosystems provides biomedical research and development services for customers seeking quantification and detection of antibodies-antigen interaction. Microplate reader assays requiring infrared (IR) or red-shifted. The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an unlimited breadth of application possibilities. Cell counters for accurate and. 価格、見積もり、お近くの販売店を. 1、卡盒式模块和细胞成像模块的构架检测功能（如FP、TRF、HTRF、AlphaScreen、ScanLater Western Blot等）卡盒模块化设计，仅通过插入卡盒就能可. Microplate Stacker. The resulting absorbance was measured at 490 nm, Spectra Max i3x (Molecular Devices, USA). 3. Using the SpectraMax i3x reader, the preconfigured SpectraMax Glo Steady-Luc Reporter assay protocol in SoftMax Pro Software was used to measure luminescence. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. 0´ 105 dsD NA (p g/ wel ) R F USpectraMax Glo Steady-Luc 工作液都加 入到微孔板后，震荡摇匀并避光放置 10 min。使用 SpectraMax Glo Steady-Luc Reporter 内置的 SpectraMax Glo Steady- Luc Reporter 实验模板设定程序并通过 SpectraMax i3x 读板机检测发光。检测结 束后，模板会根据数据自动生成一条曲 线。SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. 4 / 5. We would like to show you a description here but the site won’t allow us. ) wide and 65 cm (25. Increased fluorescence performance. 2 only) SpectraMax M-series Microplate readers. Multimode Reader Comparison Chart. Italic and boldface type are used for emphasis. Sensitive TRF detection of two simple, mix-and-measure assay workflows. Description 1. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. With optimized reagents, option to operate the readers. Video: SpectraMax i3x Overview; Brochure: SpectraMax i3x Multi-Mode Detection PlatformNORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Explore a wealth of applications with the future-ready SpectraMax i3x. We would like to show you a description here but the site won’t allow us. Fluorescence Microplate Reader Comparison Chart. 3, were mixed in a final volume of 100 μL in 10 mM sodium phosphate buffer, pH 7. StainFree Cell Detection Algorithm. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. TUNE Tunable Wavelength Detection Cartridge. Find regional support. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with. 2. 8 Antibodies and. Easily upgrade or switch between detection modes with user-exchangeable modules – just open the drawer and insert the module you want. HTRF-compatible SpectraMax Multi-Mode Readers. . Benefits • User-upgradeable application modules including cellular imaging • Sensitivity across spectrum with Spectral Fusion™ Illumination • Expanded dynamic range with cooled PMT • Control and analytics provided by SoftMax® Pro Software SpectraMax i3x The SpectraMax i3x from Molecular Devices is a multi-mode detection system that evolves with your future needs and offers an. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. 3) At the bottom of the strip, click on "Show. 22 μ m fi ltration unit (Sigma, cat. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. spectramax i3x (Danaher Inc)The fluorescence emission intensity spectra were recorded using a microplate reader (Spectra Max® i3x, Molecular Devices, LLC. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. Prepare your iD series instrument for shipment. Detection cartridge modularity allows for easy addition of new read modes within minutes without. 6% for the on-board optics (monochromators). 660-665 Eskdale Road. *On the SpectraMax i3x reader only. 312 gain calibration failed. com. Microplate assays were performed on a multimode microplate detector (Molecular Devices Spectra Max i3x). Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. i3. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Thetypeof luciferase(Renilla. Examples: fiPress carefully to engage,fl fiDo not press down. Next, an AlphaLISA assay was developed to detect levels of pro-inflammatory factors produced by endothelial cells in response to TNF-alpha stimulation. 0. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. **On the SpectraMax iD3/iD5 readers only All graphs are for illustrative purposes only. 99 to $1999. Supplier: Molecular Devices. no. Multimode Reader Comparison Chart. Page 9 Installing and Setting Up the SpectraMax MiniMax Imaging Cytometer Attaching the Imaging Cytometer to the Microplate Reader Attaching the SpectraMax MiniMax Imaging Cytometer to the SpectraMax i3x Instrument requires a work area that is approximately 130 cm (51. Quickly and easily expand the capabilities of your SpectraMax Paradigm or i3x Multi-Mode Microplate reader to include Tunable Wavelength detection using the. This instrument also provides the option of modular upgrades for Western Blotting,. Immunofluorescence StainingAfter cooled down to room temperature, the concentrations of the solution were measured by microplate reader (Spectra Max i3x) at 490 nm (Kagi and Schaffer, 1988). Analysis of HTRF assays involves Cisbio’s patented ratiometric reduction method based on the two emission wavelengths detected. *On the SpectraMax i3x reader only. Prepare your i3/i3x instrument for shipment. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate reader that evolves with your future needs and allows you to investigate every aspect of a. 4. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Afterward, the absorbance was measured at 450 nm on a Microplate Reader (Spectra Max i3x, Molecular Devices, USA). The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. Prime function 20 amol. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,Danaher Inc spectramax i3x Spectramax I3x, supplied by Danaher Inc, used in various techniques. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. 3) At the bottom of the strip, click on "Show. If you do not have an iLab account you must first make one: SpectraMax iD3 User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax iD3 Multi-Mode Microplate Reader, a versatile instrument that measures absorbance, fluorescence, and luminescence. Spectranet customer portalWatch this video to get an introduction to the Injector-Ready SpectraMax® i3x Multi-Mode Microplate Reader System. Prepare your i3/i3x instrument for shipment. Features. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. , 2011).